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. 2001 Feb 19;152(4):795–808. doi: 10.1083/jcb.152.4.795

Figure 1.

Figure 1

Rab27a colocalizes with melanosome-resident proteins in MM96 and melan-c cells. Coverslip-grown melan-c or MM96 cells were fixed and stained using the polyclonal anti-Rab27a antibody Q142 (A, D, G, and J); monoclonal antibodies reactive to the melanosomal marker proteins TRP-1 (B) or silver (E and H); and the transferrin receptor (Tfn; panel K), an endosome marker, as described in Materials and Methods. Polyclonal antibodies were detected using Alexa 488–conjugated goat anti–rabbit secondary antibodies (A, D, G, and J); monoclonal antibodies were detected using Alexa 568–conjugated goat anti–mouse second antibodies (B, E, H, and K). Merged fluorescent signals for Rab27a and the indicated marker proteins are shown in C, F, I, and L. Bar, 10 μm. See Figure S1, available at http://www.jcb.org/cgi/content/full/152/4/795/DC1.