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. 2001 Feb 19;152(4):795–808. doi: 10.1083/jcb.152.4.795

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Subcellular fractionation of melanocyte-derived membranes. Postnuclear supernatants of melan-a cells were subjected to a 28% Percoll gradient centrifugation; fractions were then collected from the top of the gradient and analyzed by immunoblot. The lightest fraction is 1 (top of the gradient) and the densest fraction is 18 (bottom of the gradient). Antibodies used are indicated on the right. Rab5, Rab6, and Rab8 localize to early endosomes, Golgi, and TGN/plasma membrane, respectively. Calnexin, the transferrin (Tfn) receptor, and tyrosinase are membrane proteins that localize to the ER, early endosomes, and melanosomes, respectively.