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. 2001 Feb 19;152(4):657–668. doi: 10.1083/jcb.152.4.657

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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GFP-Apg5 is present on isolation membranes. GFP24 (A–C, E–J) or GKR-1 (D) cells were cultured in Hanks' solution for 2 h and fixed. The localization of GFP-Apg5 was examined by silver-enhanced immunogold electron microscopy using an anti–GFP antibody. In B, an isolation membrane is enclosing a mitochondrion. The isolation membranes (arrows), autophagosomes (open arrowheads) and autolysosomes (closed arrowheads) are indicated. Double arrows indicate small membrane compartments to which GFP-Apg5 extensively localizes. The typical images of these structures are shown at higher magnification (E–J). Bars, 1 μm.