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. 2001 Feb 19;152(4):657–668. doi: 10.1083/jcb.152.4.657

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Formation of autophagosomes is traced with GFP-Apg5. (A) Sequential frames (1-min intervals) of three newly generated GFP-Apg5 spots, or a GFP-Apg5^K130R spot. GFP24 or GKR-1 cells were cultured in Hanks' solution for 1 h and directly observed by time-lapse video microscopy. Bars, 1 μm. See supplemental videos of GFP-Apg5 (videos 1–3) and GFP-Apg5^K130R (video 4). (B) Duration of each spot of GFP-Apg5 staining was measured for 15 cases that showed circularization in the same field.