Figure 1.

Development of diabetes spontaneously in HLA-DQ transgenic mice. (A) Spontaneous diabetes in DQ8 experiments. Six groups of mice were used in the study as indicated. Number of mice per group was as follows: n = 21 (10 female, 11 male) DQ8⁺/mII⁻/B7⁺; n = 15 (8 female, 7 male) DQ8⁻/mII⁻/B7⁺; n = 16 (11 female, 5 male) DQ8⁺/mII⁻/B7⁻; n = 14 (8 female, 6 male) DQ8⁻/mII⁻/B7⁻; n = 17 (6 female, 11 male) DQ8⁺/mII⁺/B7⁺; and n = 12 (9 female, 3 male) DQ8⁺/mII⁻/B7⁻. (B) Spontaneous diabetes in DQ6 experiments. Four groups of mice were used in the study as indicated. Number of mice per group was as follows: n = 16 (9 female, 7 male) DQ6⁺/mII⁻/B7⁺; n = 12 (5 female, 7 male) DQ6⁻/mII⁻/B7⁺; n = 9 (4 female, 5 male) DQ6⁺/mII⁺/B7⁺; and n = 15 (6 female, 9 male) DQ6⁺/mII⁻/B7⁻. Mice were housed under specific pathogen-free conditions. Diabetes was determined by monitoring of urinary glucose and confirmed by blood glucose (>250 mg/dl).