Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2000 Jan 3;191(1):77–88. doi: 10.1084/jem.191.1.77

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Homologous but not heterologous desensitization by CCR7 and CXCR4 ligands. Desensitization of chemotaxis experiments were performed as described (reference 22). In brief, mouse LN or spleen cells were pretreated with five times the optimal chemotactic dose of the indicated chemokine (or control medium), and washed through a serum layer to remove residual chemokine. The desensitized cells were then allowed to migrate towards the same or another chemokine (at the optimal chemotactic dose) through 5-μm pores for 15 min. Migrated cells were counted as described (references 8, 22). Percent migration of B cells was calculated based on FACS^® analysis of migrated compared with input cells; B cells were defined as CD3⁻/CD19⁺. Data shown are the mean ± SD of three experiments, each with two replicate wells per condition. Mean maximum migration of mock-treated control B cells to SDF-1α was 10%, to MIP-3β 15%, and to SLC 13%.