Figure 4.
Interactions of T and B cells with PP-HEVs of DDD/1 versus normal mice. (A) The rolling flux of injected T cells (black bars) in PP-HEVs is increased in SLC-deficient DDD/1 mice compared with age-matched normal BALB/c, while B cell (hatched bars) rolling flux is comparable. (B) T cell accumulation is severely curtailed in PP-HEVs of DDD/1 mice; B cell accumulation is similar to control BALB/c mice. Enriched T and B cell (107 cells each) populations were differentially labeled and coinjected for observation in PP-HEVs. Venular trees draining single follicles were selected for analysis. Rolling flux is the total number of rolling B or T cells observed over time and averaged for the number of venules that supported rolling in the tree. Accumulation is the average number of stuck cells of each subset in the whole tree at 8 min after injection. Data are mean results of three experimental animals of each type (± SD). Rolling velocities of T cells in DDD/1 mice were similar to those in wild-type BALB/c mice (range 43–191 μm/s, mean 99 ± 44 SD).