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. 2000 Jan 3;191(1):77–88. doi: 10.1084/jem.191.1.77

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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SLC is expressed on the lumen of PP-HEVs in normal, but not DDD/1 mice. Confocal micrographs of whole PPs from normal (left and right panels) or DDD/1 (middle panel) mice depicting localization of green anti-SLC (left and middle panels), and lack of green staining with a negative control hamster antibody (right panel); and MAdCAM-1⁺ HEVs as red fluorescence (all three panels). mAbs to SLC (anti–TCA-4, 4B1E7C10 [reference 17]) and MAdCAM-1 (MECA 89), or control antibodies to DNP (UC8-19B) and human CD44 (Hermes-1) (not shown) were conjugated to Alexa™ 488 or Alexa™ 546, respectively. 50–75 μg of anti-SLC or anti-DNP, and/or 10–20 μg anti–MAdCAM-1 or anti-huCD44 was injected via carotid artery cannulation in a single bolus. Staining was evident for both SLC and MAdCAM-1 by 1 min, maximal for MAdCAM-1 by 10 min and for SLC by 15 min. Animals were killed, and whole PPs were immediately removed for examination by confocal microscopy. Representative images (10× objective).