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. 2000 Jan 3;191(1):23–32. doi: 10.1084/jem.191.1.23

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Proliferation and differentiation of sorted pre-B I cells. CD19⁺B220⁺c-kit⁺ pre-B I cells from bone marrow (BM) and day 17 fetal liver (FL) of wild-type, bone marrow of λ₅ ^–/–, and bone marrow of bcl-2 transgenic B6 mice. (A) Viable cell recovery. (B) Cellular division as revealed by the decrease of PKH2 fluorescent intensity after 6 d (wild-type B6 bone marrow and fetal liver and bcl-2 transgenic bone marrow) or 4 d (B6 λ₅ ^−/ ⁻ bone marrow) of culture (dotted line), compared with the intensity of the starting population (solid line histogram). (C) Expression of cytoplasmic μH chain (□) and sIgM (○). (D) LPS reactivity before (○) and after (□) 6 d of culture as analyzed by limiting dilution analysis.