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. 2000 Feb 7;191(3):435–444. doi: 10.1084/jem.191.3.435

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Strongly NSE⁺ DCs are present in the LP and T cell areas of MLNs from germ-free rats. LNs and ileum from germ-free rats were snap frozen. Cryosections of ileum (A) and MLN (B) were fixed in 2% paraformaldehyde, reacted for NSE (red), and labeled for OX62 (blue in A, brown in B). (A) Double-positive cells (<) are present in apposition to the epithelium. One cell (<<, enlarged in inset) appears to be engulfing an epithelial cell. (B) Several large, irregular, double-positive cells are present in the T cell area of the node (<, >).