Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2000 Feb 7;191(3):475–484. doi: 10.1084/jem.191.3.475

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Levels of CD38 on antigen-specific IgG1 B cells at day 42 of the primary response. (A) Spleens from mice immunized 42 d previously with 100 μg i.p. of NP–KLH were stained with the indicated antibodies and analyzed by flow cytometry. Viable cells having the phenotype IgM⁻IgD⁻ were electronically gated on (rectangle) and examined for expression of IgG1 and the ability to bind the immunizing hapten NP coupled to a fluorescent protein. Such double-positive cells were gated on (rectangle), and the level of CD38 was determined. This result is depicted as the solid histogram in this figure. Negative control staining was less than the fluorescence level marked by the dashed vertical line. The percentages of NP-binding IgG1⁺ cells expressing high levels of CD38 are indicated. Cells used in this experiment were pooled from three mice, and the data shown are representative of three experiments. (B) Germinal center cells in bcl-2–transgenic mice downregulate CD38. Mesenteric LN B cells were gated on by expression of CD45R. These cells were then examined for CD38 levels and binding of the lectin peanut agglutinin; such B cells are boxed.

Levels of CD38 on antigen-specific IgG1 B cells at day 42 of the primary response. (A) Spleens from mice immunized 42 d previously with 100 μg i.p. of NP–KLH were stained with the indicated antibodies and analyzed by flow cytometry. Viable cells having the phenotype IgM⁻IgD⁻ were electronically gated on (rectangle) and examined for expression of IgG1 and the ability to bind the immunizing hapten NP coupled to a fluorescent protein. Such double-positive cells were gated on (rectangle), and the level of CD38 was determined. This result is depicted as the solid histogram in this figure. Negative control staining was less than the fluorescence level marked by the dashed vertical line. The percentages of NP-binding IgG1⁺ cells expressing high levels of CD38 are indicated. Cells used in this experiment were pooled from three mice, and the data shown are representative of three experiments. (B) Germinal center cells in bcl-2–transgenic mice downregulate CD38. Mesenteric LN B cells were gated on by expression of CD45R. These cells were then examined for CD38 levels and binding of the lectin peanut agglutinin; such B cells are boxed.