Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2000 Feb 7;191(3):475–484. doi: 10.1084/jem.191.3.475

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Reduced frequency of V_H gene somatic mutations in NP-specific memory B cells from bcl-2–transgenic mice. The frequency of mutations in V_H186.2 genes from single, antigen-specific CD38⁺IgG1⁺ B cells was determined by nucleotide sequencing. Single cells were sorted from a pooled spleen preparation from each mouse strain using the criteria depicted in Fig. 1. All recovered sequences showed unique CDR3 junctions, indicating clonality. (B) Cumulative distribution of somatic mutations in control and bcl-2 NP-specific B cells. In both components of the figure, the number of sequences containing mutations giving rise to a tryptophan→leucine exchange at amino acid 33 (numbered according to reference 24) is depicted by the hatched segment of each column. Details of the sequences are summarized in Table . These sequence data are available from EMBL/GenBank/DDBJ under accession no. AF210258-AF210307.

Reduced frequency of V_H gene somatic mutations in NP-specific memory B cells from bcl-2–transgenic mice. The frequency of mutations in V_H186.2 genes from single, antigen-specific CD38⁺IgG1⁺ B cells was determined by nucleotide sequencing. Single cells were sorted from a pooled spleen preparation from each mouse strain using the criteria depicted in Fig. 1. All recovered sequences showed unique CDR3 junctions, indicating clonality. (B) Cumulative distribution of somatic mutations in control and bcl-2 NP-specific B cells. In both components of the figure, the number of sequences containing mutations giving rise to a tryptophan→leucine exchange at amino acid 33 (numbered according to reference 24) is depicted by the hatched segment of each column. Details of the sequences are summarized in Table . These sequence data are available from EMBL/GenBank/DDBJ under accession no. AF210258-AF210307.