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. 2000 Feb 21;191(4):613–624. doi: 10.1084/jem.191.4.613

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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DCs process residual 14028 and CS022 expressing Crl-OVA for OVA peptide presentation on MHC-I and MHC-II equally well. Logarithmically growing (OD₆₀₀ = 1.3–1.5) 14028r Crl-OVA or CS022r Crl-OVA bacteria were treated with 100 μg/ml of gentamycin (a and b), heat killed (HK) at 65°C (c and d), or were left untreated (e and f) before coincubation with DCs for 90 min. After several washes, OVA(257–264)/K^b (a, c, and e) and OVA(265–277)/I-A^b (b, d, and f) were quantitated using CD8OVA and OT4H.2D5 T hybridoma cells, respectively. The results are representative of three independent experiments.