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Inhibition of FMLP-mediated chemotaxis by N-t-BOC. A total of 5 × 105 purified human neutrophils was placed in the upper well of a chemotaxis chamber with various concentrations of N-t-BOC (0 to 800 μM) and an antagonist of the high-affinity FPR. FMLP (50 nM) was placed in the lower chamber. Greater than 95% inhibition of FMLP-mediated chemotaxis occurred with the use of concentrations of N-t-BOC that were greater than 400 μM.
