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. 2000 Mar 6;191(5):781–794. doi: 10.1084/jem.191.5.781

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Expression of SHIP in B cell subsets. (A) Southern blots of amplified total cDNA prepared from phenotypically defined bone marrow and spleen B cell subsets, as well as total bone marrow (TBM), were sequentially hybridized with probes specific for SHIP and actin as indicated. (B) Signal intensities after hybridization were quantified by PhosphorImager^® analysis, and expression levels of SHIP relative to actin were calculated. Average expression levels from two determinations are shown normalized to expression in the B220⁺c-kit⁺ population.

Expression of SHIP in B cell subsets. (A) Southern blots of amplified total cDNA prepared from phenotypically defined bone marrow and spleen B cell subsets, as well as total bone marrow (TBM), were sequentially hybridized with probes specific for SHIP and actin as indicated. (B) Signal intensities after hybridization were quantified by PhosphorImager^® analysis, and expression levels of SHIP relative to actin were calculated. Average expression levels from two determinations are shown normalized to expression in the B220⁺c-kit⁺ population.

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