Table 3.
FACS® Analysis of Selected Tissues from NOD/ SCID Mice Transplanted with Wild-Type or SHIP−/− Bone Marrow Cells
| Percentage of positive cells | ||
|---|---|---|
| +/+ | −/− | |
| Bone marrow | ||
| Ly5.2+ | 44.5 ± 11.5 | 59.6 ± 16.3 |
| B220+ | 14.8 ± 1.1 | 10.2 ± 4.0 |
| CD43+B220+ | 3.0 ± 0.9 | 2.6 ± 0.1 |
| IgM+B220lo | 3.1 ± 0.1 | 1.5 ± 1.0 |
| IgM+B220hi | 3.5 ± 1.1 | 0.6 ± 0.3 |
| Spleen | ||
| Ly5.2+ | 85.5 ± 9.6 | 61.9 ± 5.1 |
| B220+ | 54.5 ± 11.3 | 35.2 ± 1.1 |
| IgM+IgD+ | 50.0 ± 8.5 | 29.1 ± 4.4 |
| Mac-1+Gr-1+ | 8.8 ± 0.5 | 12.5 ± 0.3 |
| Lymph nodes | ||
| Ly5.2+ | 99.3 ± 0.1 | 86.2 ± 12.7 |
| B220+ | 36.1 ± 0.1 | 40.7 ± 6.1 |
| IgM+IgD+ | 32.4 ± 1.6 | 33.0 ± 7.5 |
| Mac-1+Gr-1+ | 12.4 ± 8.4 | 3.0 ± 0.7 |
Single cell suspensions, prepared from the various tissues, were labeled with the indicated antibodies as described in Materials and Methods, and were analyzed on a FACStarPLUS™. Values represent the mean ± SD for two determinations. Statistical analysis was carried out using the Student's t test.