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. 2000 Mar 6;191(5):805–812. doi: 10.1084/jem.191.5.805

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Allorestricted CTL lines from bm1 mice against K^b binding self-peptides. (a) Self-peptides eluted from K^b and used in this study as mixture K^b _(self) (references 12, 43, 55, 59, 60; and this study). (b) Splenocytes from bm1 animals were plated in limiting dilution and stimulated with TAP-1^−/− cells in the presence of a mixture of self-peptides at 500 (left), 100 (middle), and 50 ng/ml (right). After 10 d, the cultures were assayed against RMA.S in the absence or presence of the mixture. Spontaneous release was <10%. (c) Peptide specificity of expanded CTL lines. All peptides listed in a were titrated on ⁵¹Cr-labeled RMA.S cells. The indicated effector cells were added at effector to target ratios between 2 and 5. (d) The expanded CTL lines recognize tumor cells and Con A–induced blasts from B6, but not from bm1 mice. Spontaneous release was <10% for tumor cells and <18% for blasts.

Allorestricted CTL lines from bm1 mice against K^b binding self-peptides. (a) Self-peptides eluted from K^b and used in this study as mixture K^b _(self) (references 12, 43, 55, 59, 60; and this study). (b) Splenocytes from bm1 animals were plated in limiting dilution and stimulated with TAP-1^−/− cells in the presence of a mixture of self-peptides at 500 (left), 100 (middle), and 50 ng/ml (right). After 10 d, the cultures were assayed against RMA.S in the absence or presence of the mixture. Spontaneous release was <10%. (c) Peptide specificity of expanded CTL lines. All peptides listed in a were titrated on ⁵¹Cr-labeled RMA.S cells. The indicated effector cells were added at effector to target ratios between 2 and 5. (d) The expanded CTL lines recognize tumor cells and Con A–induced blasts from B6, but not from bm1 mice. Spontaneous release was <10% for tumor cells and <18% for blasts.