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. 2000 Mar 6;191(5):771–780. doi: 10.1084/jem.191.5.771

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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IL-15^−/− mice have normal numbers of conventional T cells but reduced numbers of thymic NK T cells. (A) Thymocytes from individual male IL-15^+/− (left) or IL-15^−/− (right) littermates were analyzed for expression of CD4 and CD8 (n = 2/group; 12–13 wk of age). The numbers shown represent the percentages of cells within each quadrant. The thymic cellularity was similar in both groups. The results shown are representative of three experiments. (B) NK T cell populations of thymocytes (top), intrahepatic mononuclear cells (middle), and splenocytes (bottom) from individual C57BL/6 or IL-15^−/− mice. Live gating of the HSA^lowCD8^low thymocyte population was used to acquire data on CD44^hiNK1.1⁺ or CD44^hiTCR Vβ8⁺ thymocytes. Similarly, live gating of B220^null splenocytes was used to acquire data on NK1.1⁺TCR-β⁺ splenic NK T cells. The numbers shown represent the percentage of cells with an NK T cell phenotype. (C) Absolute numbers of thymic NK T populations in control and IL-15^−/− mice. The numbers shown represent the mean absolute number ± SEM of thymic NK T cell populations from C57BL/6 or IL-15^−/− mice (n = 3/group). NK T cell numbers were calculated from the percentages of HSA^low CD8^low thymocytes that were Ly6C^hi NK1.1⁺ or CD44^hi and NK1.1⁺, TCR-α/β⁺, or Vβ8.1,8.2⁺. The results shown are representative of two experiments.

IL-15^−/− mice have normal numbers of conventional T cells but reduced numbers of thymic NK T cells. (A) Thymocytes from individual male IL-15^+/− (left) or IL-15^−/− (right) littermates were analyzed for expression of CD4 and CD8 (n = 2/group; 12–13 wk of age). The numbers shown represent the percentages of cells within each quadrant. The thymic cellularity was similar in both groups. The results shown are representative of three experiments. (B) NK T cell populations of thymocytes (top), intrahepatic mononuclear cells (middle), and splenocytes (bottom) from individual C57BL/6 or IL-15^−/− mice. Live gating of the HSA^lowCD8^low thymocyte population was used to acquire data on CD44^hiNK1.1⁺ or CD44^hiTCR Vβ8⁺ thymocytes. Similarly, live gating of B220^null splenocytes was used to acquire data on NK1.1⁺TCR-β⁺ splenic NK T cells. The numbers shown represent the percentage of cells with an NK T cell phenotype. (C) Absolute numbers of thymic NK T populations in control and IL-15^−/− mice. The numbers shown represent the mean absolute number ± SEM of thymic NK T cell populations from C57BL/6 or IL-15^−/− mice (n = 3/group). NK T cell numbers were calculated from the percentages of HSA^low CD8^low thymocytes that were Ly6C^hi NK1.1⁺ or CD44^hi and NK1.1⁺, TCR-α/β⁺, or Vβ8.1,8.2⁺. The results shown are representative of two experiments.