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. 2000 Oct 16;192(8):1191–1196. doi: 10.1084/jem.192.8.1191

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Strategy for the discrimination of D-Jβ and V-DJβ gene rearrangements. The top line drawing is a scale representation of the D-J-C region of the murine TCR-β locus, spanning 17 kb of DNA. White boxes show the location of coding sequences, as indicated; the probes used are indicated by hatched boxes. Relevant restriction fragments produced by ApaLI-SacI or ApaLI-ClaI digestion are indicated by bold lines. The bottom part of the figure illustrates the restriction fragments produced after D-J or V-DJ rearrangement. Dashed diagonal lines indicate intervening sequences deleted during D-J rearrangement, which produce several progressively smaller fragments flanked by germline restriction sites. The circular product (not drawn to scale) illustrates the nongermline fragment produced by V-DJ rearrangement; this fragment is flanked on one end by the original (germline) ApaLI site, as well as by a de novo ApaLI site formed by the ligation of Dβ (open triangle) and Vβ (filled triangle) RSS.