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. 2000 Oct 16;192(8):1151–1164. doi: 10.1084/jem.192.8.1151

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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LM-PCR identifies dsDNA fragments generated by cleavage at the 5′ and 3′ heptamers. Genomic DNA was prepared from CD19⁺ cells from two synovial tissue samples (lanes 1 and 2) and from human bone marrow (lane 3) by the agarose plug method (reference 53). LM-PCR was then used to identify blunt-ended dsDNA breaks (reference 55). The V_H-containing products of these reactions were visualized with ethidium bromide staining. The 320- and 249-bp bands contained DNA fragments with the linker attached at or in the 5′ (320 bp) and 3′ (249 bp) heptamers. Lane 4 is a template-deprived negative control. M, marker lane.