Table 1.
Molecular Characterization of the RA Synovial Tissue B Cell Clones That Underwent VH Replacement
| Type of diversification | No. of mutations | |||||||
|---|---|---|---|---|---|---|---|---|
| Clone | Source | No. of identicalsequences | VH gene(s) used | Ig isotype | VH mutation | VH replacement | FRR/S | CDRR/S |
| ST1R M5 | cDNA | 1 | VH 1–58 | IgM | − | − | 0/0 | 0/0 |
| ST1R M1 | cDNA | 1 | VH 1–58 | IgM | + | − | 0/1 | 0/0 |
| ST1R M8 | cDNA | 1 | VH 1–58 | IgM | + | − | 0/1 | 0/0 |
| ST1R M26 | cDNA | 1 | VH 1–58 | IgM | + | − | 1/0 | 0/0 |
| ST1R M6 | cDNA | 1 | VH 1–58/VH 1–18 | IgM | + | + | 5/2 | 1/0 |
| ST1R M31 | cDNA | 1 | VH 1–58/VH 1–24 | IgM | + | + | 1/2 | 0/0 |
| ST1R M10 | cDNA | 1 | VH 1–58/VH 1–69 | IgM | + | + | 10/5 | 4/1 |
| ST1R M17 | cDNA | 1 | VH 1–08 | IgM | + | − | 4/4 | 4/1 |
| ST1R M9 | cDNA | 1 | VH 1–08/VH 1–69 | IgM | + | + | 0/1 | 0/0 |
| ST1R G1 | cDNA | 1 | VH 3–09/VH 1–02 | IgG | + | + | 17/3 | 18/1 |
| ST1R G2 | cDNA | 1 | VH 3–09/VH 1–69 | IgG | + | + | 11/6 | 3/2 |
| ST1L G12 | cDNA | 1 | VH 1–69 | IgG | + | − | 8/3 | 2/1 |
| ST1L G2 | cDNA | 8 | VH 1–69 | IgG | + | − | 7/4 | 2/1 |
| ST1L G32 | cDNA | 1 | VH 1–69 | IgG | + | − | 7/4 | 2/2 |
| ST1L G22 | cDNA | 1 | VH 1–69 | IgG | + | − | 9/4 | 2/1 |
| ST1L G11 | cDNA | 1 | VH 1–69 | IgG | + | − | 9/5 | 5/1 |
| ST1L G19 | cDNA | 1 | VH 1–69/VH 1–08 | IgG | + | + | 10/6 | 4/1 |
| ST1L G8 | cDNA | 1 | VH 1–08 | IgG | + | − | 10/5 | 4/1 |
| ST1L G27 | cDNA | 1 | VH 1–08 | IgG | + | − | 13/4 | 4/1 |
| ST1L G29 | cDNA | 1 | VH 1–08/VH 1–69 | IgG | + | + | 7/2 | 3/1 |
| ST2 14 | DNA | 5 | VH 1–46 | Indeterminate | − | − | 0/0 | 0/0 |
| ST2 10 | DNA | 1 | VH 1–46/VH 1–02 | Indeterminate | + | + | 5/3 | 6/2 |
| ST2 6 | DNA | 2 | VH 1–58 | Indeterminate | + | − | 0/0 | 1/0 |
| ST2 31 | DNA | 1 | VH 1–18/VH 1–46 | Indeterminate | + | + | 3/1 | 0/0 |
| ST2 2 | DNA | 2 | VH 1–46 | Indeterminate | − | − | 0/0 | 0/0 |
| ST2 26 | DNA | 1 | VH 1–46/VH 1–69 | Indeterminate | + | + | 6/2 | 0/0 |
The nucleotide sequences of 95 cDNA clones and 36 genomic DNA clones were determined. Only those clones that contained progeny exhibiting VH replacement are listed. R, replacement; S, silent.