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Activation via the BCR and CD40 induces strong expression of HSPGs on B cells. Tonsillar B cells were cultured on CD40L-transfected L cells or wild-type L cells, in the presence or absence of BCR stimuli, i.e., anti-Ig immunobeads (Ig) or SAC, for 0, 24, or 48 h, and analyzed by FACS®. Expression of HSPGs is given as the mean fluorescence intensity of the anti–ΔHS-stub staining after heparitinase treatment minus its staining before treatment.
