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. 2000 Oct 16;192(8):1197–1204. doi: 10.1084/jem.192.8.1197

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Inflammatory exudates from murine dorsal pouches treated with aspirin generate novel compounds: LC/MS/MS. TNF-α–induced leukocyte exudates collected at 6 h from FVB mice given ASA (3.5 h at 500 μg/air pouch) and EPA (4 h at 300 μg/pouch) contained (2.3 ± 0.5 × 10⁶ leukocytes per pouch); see Materials and Methods. (A) Selected ion chromatogram of mono-HEPEs. (B) MS/MS of 18R-HEPE. (C) MS/MS of 5S-HEPE. (D) MS/MS of 12,15,18R-triHEPE (see text for identification of diagnostic ions). Results are representative of n = 4.