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. 2000 Oct 16;192(8):1197–1204. doi: 10.1084/jem.192.8.1197

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Human ECs and PMNs: novel trihydroxy-containing compounds LC/MS/MS. (A) HUVECs treated with IL-1β (24 h) and ASA. (B) Human PMNs (∼ 30 × 10⁶/ml) incubated (30 min, 37°C) with serum-treated zymosan (100 ng/ml) and acetylated ASA-recombinant COX-2–derived products from EPA. (C) MS/MS of 5,12,18R-triHEPE (see text for ions and fragmentation). (D) MS/MS of 15-epi-LXA₅. Results are representative of n = 3–5 for PMNs and n = 3 for ECs.