Figure 8.

Mice given OT1 T cells do not clear antigen-bearing DCs more rapidly than nontransferred mice. B6.PL mice with and without transfer of 2.5 × 10⁵ OT1 T cells were challenged with increasing numbers of UBI-GFP bone marrow DCs pulsed with 5 ng/ml of ova8 peptide. DCs from the UBI-GFP mice were cultured as in the legend to Fig. 6. 5 d later, splenic DCs were isolated by collagenase treatment and assessed for the presence of GFP⁺ events. (A) GFP histograms of mice with and without transfer of OT1 cells. All GFP⁺ cells were also class II⁺ (not shown). (B) Percentage of GFP⁺ events of total spleen cells as shown in A, expressed as the average and standard deviation from three mice per group.