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. 2000 Oct 16;192(8):1105–1114. doi: 10.1084/jem.192.8.1105

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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OT1 T cells compete with endogenous T cells for access to DCs. B6.PL mice with and without the transfer of 2.5 × 10⁵ OT1 cells were challenged intravenously with B6 bone marrow DCs pulsed with 5 ng/ml of ova8 peptide. 5 d later, spleen cells were stained with tetramer as in the legend to Fig. 2. (A) Nontransferred B6.PL response to increasing numbers of DCs injected. The dot plots are gated on CD8⁺class II⁻ events. The histograms were further gated on Thy1.2⁻ (gate R3) events. (B) OT1-transferred B6.PL response to increasing numbers of DCs injected. Gating strategy as in A. (C) The average and standard deviation of endogenous T cell percentages from OT1-transferred and nontransferred mice, three mice per treatment.