Figure 2.
(A) SPR binding curve for isoalloxazine 1a to c-kit2 (●), c-kit1 (▲), and htelo (■); running buffer, 50 mM Tris-HCl pH 7.4, 100 mM KCl. (B) FRET-melting assay for c-kit1 (∗), htelo (■), c-kit2 (●), and ds DNA (▲) in the presence of 1a; buffer, 60 mM potassium cacodylate pH 7.4.