Figure 2.

Impaired survival in vitro and increased turnover in vivo of IKKα^2/− B cells. (A and B) PB and splenocytes from IKKα^1/+ and IKKα^2/− RAG2-deficient B6 chimeras were cultured in complete RPMI 1640 in the absence or presence of mitogens for the indicated periods and stained with FITC–annexin V and PE-B220. Percentages of annexin⁺ B cells were calculated by dividing percentages of B220⁺annexin⁺ cells by those of total B (B220⁺annexin⁺ and B220⁺annexin⁻) cells and shown as bar graphs in B. (C) Increased B cell turnover in IKKα^2/− chimeras. The turnover of splenic B cells was determined by BrdU incorporation. Numbers represent percentages of the quadrants. Experiments were independently performed three times with similar results. One representative experiment is shown.