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. 2001 Feb 19;193(4):417–426. doi: 10.1084/jem.193.4.417

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Impaired mitogenic responses of IKKα^2/− B cells. (A) Splenic B cells from IKKα^1/+ and IKKα^2/− RAG2-deficient B6 chimeras were cultured in the absence or presence of mitogens for 72 h and analyzed for their [³H]thymidine incorporation. The data indicate means ± SD of triplicate samples of one representative experiment. (B) Splenocytes from IKKα^1/+ and IKKα^2/− RAG2-deficient B6 chimeras were cultured with LPS or anti-CD40 for 48 h. Forward scatter (FSC) and side scatter (SSC) of harvested cells were analyzed by FACS^®. Blasts are indicated by enclosed areas. Experiments were independently performed three times with similar results. One representative experiment is shown.