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. 2001 Feb 19;193(4):471–482. doi: 10.1084/jem.193.4.471

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© 2001 The Rockefeller University Press

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DSS-induced colitis in IL-6^−/− and IFN-γ^−/− mice. (A) Time course of body weight loss. WT C57BL6 mice or IL-6^−/− or IFN-γ^−/− mice (n = 3 for each knockout mice) were treated with 2% DSS for indicated periods, and body weight was measured daily. Relative body weight (%) compared with that of day 0 is plotted with a standard error. Results were analyzed using the t test. A P value of <0.05 was considered to be statistically significant. (B) Histological section of an ulcer with hematoxylin and eosin staining. Pathology was graded on a scale of 0–12. Data represent the average with SEM for the group. The average score for IL-6^−/− mice, but not IFN-γ^−/− mice, was significantly different from that for the WT mice (P = 0.013). Statistical analysis was performed using the t test. (C) STAT3 phosphorylation in the colon of WT and IL-6^−/− mice treated with DSS. Colon tissue extracts from three independent mice of each strain were immunoblotted with anti–phospho-STAT3 (αPY-STAT3) and anti-STAT3 (αSTAT3) antibodies.

DSS-induced colitis in IL-6^−/− and IFN-γ^−/− mice. (A) Time course of body weight loss. WT C57BL6 mice or IL-6^−/− or IFN-γ^−/− mice (n = 3 for each knockout mice) were treated with 2% DSS for indicated periods, and body weight was measured daily. Relative body weight (%) compared with that of day 0 is plotted with a standard error. Results were analyzed using the t test. A P value of <0.05 was considered to be statistically significant. (B) Histological section of an ulcer with hematoxylin and eosin staining. Pathology was graded on a scale of 0–12. Data represent the average with SEM for the group. The average score for IL-6^−/− mice, but not IFN-γ^−/− mice, was significantly different from that for the WT mice (P = 0.013). Statistical analysis was performed using the t test. (C) STAT3 phosphorylation in the colon of WT and IL-6^−/− mice treated with DSS. Colon tissue extracts from three independent mice of each strain were immunoblotted with anti–phospho-STAT3 (αPY-STAT3) and anti-STAT3 (αSTAT3) antibodies.