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. 2001 Feb 19;193(4):471–482. doi: 10.1084/jem.193.4.471

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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The effect of F59D-JAB on STAT3 repression by WT JAB and CIS3 in 293 cells. (A) 293 cells were transfected with 10 ng WT JAB or CIS3, APRE-luciferase reporter gene, and indicated amounts of F59D-JAB plasmid (ng). 2 d after transfection, LIF-induced STAT3 activation was measured by luciferase assay. (B) 293 cells were transfected with 100 ng Myc-tagged WT JAB or CIS3 and hemagglutinin (HA)-tagged STAT3 with indicated amounts of F59D-JAB. After stimulation with 100 ng/ml LIF for 30 min, cell extracts were immunoprecipitated with anti-HA, and then the immunoprecipitates (αHA-IP) were blotted with anti-phosphorylated STAT3 (αPY-STAT3) or anti-STAT3 (αSTAT3). Total cell extracts (TCL) were blotted with anti-Myc (αMyc) and anti-JAB (αJAB) antibodies to confirm the expression of Myc-JAB, Myc-CIS3, and F59D-JAB.