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. 2001 Feb 5;193(3):281–296. doi: 10.1084/jem.193.3.281

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Enhanced IgM binding to F62_PorMS11: Por influences IgM binding to gonococci. Despite the ability of F62_PorMS11 to bind C4bp (see Fig. 2), the resulting transformant possessed the SS phenotype on bactericidal testing (100% killing in 10% NHS at 30 min). To determine the mechanism of serum sensitivity, we studied the binding of IgM, C3, C5b-9, and C4bp to F62_PorMS11 (gray shaded areas) in comparison with F62 (dotted lines) and F62_loop1MS11_loop2–8 (solid lines). In contrast to F62_PorMS11, F62_loop1MS11_loop2–8 was fully SR (100% survival in 10% NHS at 30 min), and differed from the former only at Por loop 1. F62_PorMS11 bound two- and fourfold more IgM than F62 and F62_loop1MS11_loop2–8, respectively. Levels of C3 and C5b-9 binding to F62_PorMS11 were intermediate compared with the two other strains. C4bp binding to F62_PorMS11 and F62_loop1MS11_loop2–8 were identical.