. 2001 Feb 5;193(3):281–296. doi: 10.1084/jem.193.3.281

Table 3.

Primer Pairs Used for Constructing MS11-F62 Hybrid Por Molecules Using Overlap Extension PCR

Mutation	Primer sequence (5′→3′)
F62₅ to MS11₅	GGGATACTATAAACTTGATGTTCGTATTCGATTTTTTTAGTG/PorI
	CGAATACGAACATCAAGTTTATAGTATCCCCAGCCTGTTTGT/PorII
F62₆ to MS11₆	TCACGCACTAATTGATTTTGATACAATTTGGCATCTTGTTG/PorI
	TTGTATCAAAATCAATTAGTGCGTGATAATTCGCACAACTCTCAA/PorII
MS11₅ to F62₅	AAACTGGGGATATTATATGCATATCCTTCCATTTTTTTAGTGCCTTCGCCG/PorI
	AAAAATGGAAGGATATGCATATAATATCCCCAGTTTGTTTGTTTGTTGAAAA/PorII
MS11₆ to F62₆	GCACGCCATGTTCCATACAATTTGGCATCTTGTTGTTGTG/PorI
	CACAACAACAAGATGCCAAATTGTATGGAACATGGCGTGC/PorII