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. 2001 Feb 5;193(3):317–328. doi: 10.1084/jem.193.3.317

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© 2001 The Rockefeller University Press

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Inefficient proliferation and IL-2 production by T cells lacking JNK1. (a–c) Cells from (a) spleen, (b) lymph nodes, or (c) purified splenic T cells, all from 4–8-wk-old Jnk1 ^+/+ or Jnk1^−/− mice, were cultured in the presence of the indicated concentrations of plate-bound anti-CD3ε antibody in the absence or presence of the indicated amounts of anti-CD28 antibody. After 60 h, cells were pulsed for 12 h with 1 μCi ^[3H]thymidine/well and collected for measurement of [³H]thymidine incorporation into DNA. (d) IL-2 production was measured by ELISA on media collected 24 h after stimulation of purified splenic T cells as described above. (e) Proliferation rates of purified T cells cultured in the presence of 50 U/ml IL-2 plus anti-CD3 and/or anti-CD28 antibodies. Proliferation rates of splenic B cells cultured in medium alone (untreated), or in the presence of either 50 U/ml IL-4 plus 1 μg/ml soluble anti-CD40 or 10 μg/ml LPS. The results are representative of four independent experiments, each using three matched pairs of mice. All assays were conducted in triplicates. Standard deviations are shown as vertical lines.

Inefficient proliferation and IL-2 production by T cells lacking JNK1. (a–c) Cells from (a) spleen, (b) lymph nodes, or (c) purified splenic T cells, all from 4–8-wk-old Jnk1 ^+/+ or Jnk1^−/− mice, were cultured in the presence of the indicated concentrations of plate-bound anti-CD3ε antibody in the absence or presence of the indicated amounts of anti-CD28 antibody. After 60 h, cells were pulsed for 12 h with 1 μCi ^[3H]thymidine/well and collected for measurement of [³H]thymidine incorporation into DNA. (d) IL-2 production was measured by ELISA on media collected 24 h after stimulation of purified splenic T cells as described above. (e) Proliferation rates of purified T cells cultured in the presence of 50 U/ml IL-2 plus anti-CD3 and/or anti-CD28 antibodies. Proliferation rates of splenic B cells cultured in medium alone (untreated), or in the presence of either 50 U/ml IL-4 plus 1 μg/ml soluble anti-CD40 or 10 μg/ml LPS. The results are representative of four independent experiments, each using three matched pairs of mice. All assays were conducted in triplicates. Standard deviations are shown as vertical lines.