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. 2001 Feb 5;193(3):317–328. doi: 10.1084/jem.193.3.317

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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JNK activity and c-Jun status in Jnk1 ⁻ ^/− cells. (a) Thymocytes and (b) lymph node cells from wild-type and Jnk1 ⁻ ^/− mice were stimulated for the indicated times with 10 μg/ml anti-CD3 plus 0.1 μg/ml anti-CD28 antibodies, and JNK activity was determined in whole cell extracts, using solid-state kinase assay and GST–c-Jun (1-79) as a substrate. The same extracts were analyzed for their content of phospho-c-Jun (S63), total c-Jun, and actin by Western blot analysis.