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. 2001 Feb 5;193(3):329–338. doi: 10.1084/jem.193.3.329

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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CD4⁺ AND transgenic T cells from MRL mice proliferate more after agonist peptide and mitogenic stimulation than CD4⁺ AND transgenic T cells from nonautoimmune strains. (A) Naive CD4⁺ transgenic T cells were labeled with CFSE and stimulated with 0.05 μM PCC 88–104 agonist peptide using CH27 cells as APCs. The number of cell divisions at 72 h was determined by CFSE labeling. CH27 and dead T cells were excluded from the analysis on the basis of forward/side scatter. The mean number of cell divisions at 72 h is indicated in the bar graph at the right. *P < 0.005 for comparison of MRL.AND to B10.AND or CBA.AND cells. Arrows indicate evidence of cell division. (B) Naive AND transgenic T cells were stimulated in vitro by I-E^k bearing B7.1⁺/B7.2⁺ CH27 cells pulsed with varying concentrations of PCC 88–104. Proliferation was measured at 72 h by [³H]thymidine incorporation. Results are means of quadruplicate measurements. Error bars represent SEM. *P < 0.05 for comparison of MRL.AND to B10.AND or CBA.AND cells. (C) Naive AND transgenic T cells were stimulated in vitro with 10 ng/ml of PMA and 500 ng/ml of ionomycin. Proliferation was measured at 72 h by [³H]thymidine incorporation. Results are means of quadruplicate measurements. *P < 0.05 for comparison of MRL.AND to B10.AND or CBA.AND cells.

CD4⁺ AND transgenic T cells from MRL mice proliferate more after agonist peptide and mitogenic stimulation than CD4⁺ AND transgenic T cells from nonautoimmune strains. (A) Naive CD4⁺ transgenic T cells were labeled with CFSE and stimulated with 0.05 μM PCC 88–104 agonist peptide using CH27 cells as APCs. The number of cell divisions at 72 h was determined by CFSE labeling. CH27 and dead T cells were excluded from the analysis on the basis of forward/side scatter. The mean number of cell divisions at 72 h is indicated in the bar graph at the right. *P < 0.005 for comparison of MRL.AND to B10.AND or CBA.AND cells. Arrows indicate evidence of cell division. (B) Naive AND transgenic T cells were stimulated in vitro by I-E^k bearing B7.1⁺/B7.2⁺ CH27 cells pulsed with varying concentrations of PCC 88–104. Proliferation was measured at 72 h by [³H]thymidine incorporation. Results are means of quadruplicate measurements. Error bars represent SEM. *P < 0.05 for comparison of MRL.AND to B10.AND or CBA.AND cells. (C) Naive AND transgenic T cells were stimulated in vitro with 10 ng/ml of PMA and 500 ng/ml of ionomycin. Proliferation was measured at 72 h by [³H]thymidine incorporation. Results are means of quadruplicate measurements. *P < 0.05 for comparison of MRL.AND to B10.AND or CBA.AND cells.