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. 2001 Feb 5;193(3):329–338. doi: 10.1084/jem.193.3.329

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© 2001 The Rockefeller University Press

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CD4⁺ AND transgenic T cells from MRL mice proliferate more and differentially express activation markers in response to low affinity TCR ligands compared with CD4⁺ transgenic T cells from nonautoimmune mice. (A and B) Naive splenic CD4⁺ transgenic T cells were stimulated with varying concentrations of K99Q (A) or A96I (B) presented by I-E^k bearing B7.1⁺/B7.2⁺ CH27 cells. (C) Naive CD44^loCD62L^hi AND transgenic T cells from lymph nodes were stimulated with varying concentrations of K99R presented by CH27 cells. Proliferation was measured at 72 h by [³H]thymidine incorporation. Results are means of quadruplicate measurements. *P < 0.005 for comparison of MRL.AND to B10.AND or CBA.AND cells in A–C. (D) Naive CD44^loCD62L^hi splenic CD4⁺ transgenic T cells were stimulated with 25 μM K99Q or 0.05 μM PCC 88–104 presented by CH27 cells. CD44 and CD62L expression were determined by flow cytometry 48 h after stimulation, with CD44^hi cells designated as activated cells and CD44^hiCD62L^lo cells as memory cells. Arrows indicate the population of activated T cells that have upregulated CD44.

CD4⁺ AND transgenic T cells from MRL mice proliferate more and differentially express activation markers in response to low affinity TCR ligands compared with CD4⁺ transgenic T cells from nonautoimmune mice. (A and B) Naive splenic CD4⁺ transgenic T cells were stimulated with varying concentrations of K99Q (A) or A96I (B) presented by I-E^k bearing B7.1⁺/B7.2⁺ CH27 cells. (C) Naive CD44^loCD62L^hi AND transgenic T cells from lymph nodes were stimulated with varying concentrations of K99R presented by CH27 cells. Proliferation was measured at 72 h by [³H]thymidine incorporation. Results are means of quadruplicate measurements. *P < 0.005 for comparison of MRL.AND to B10.AND or CBA.AND cells in A–C. (D) Naive CD44^loCD62L^hi splenic CD4⁺ transgenic T cells were stimulated with 25 μM K99Q or 0.05 μM PCC 88–104 presented by CH27 cells. CD44 and CD62L expression were determined by flow cytometry 48 h after stimulation, with CD44^hi cells designated as activated cells and CD44^hiCD62L^lo cells as memory cells. Arrows indicate the population of activated T cells that have upregulated CD44.