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. 2001 Feb 5;193(3):329–338. doi: 10.1084/jem.193.3.329

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© 2001 The Rockefeller University Press

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MRL.AND transgenic T cells produce more IL-2 after stimulation with APLs than control transgenic T cells. (A) Negatively selected naive CD4⁺ AND transgenic T cells were stimulated in vitro with varying concentrations of a low affinity APL of PCC 88–104 (K99Q) presented by CH27 cells. IL-2 was determined by indirect ELISA from culture supernatant 24 h after stimulation. Error bars represent SEM of triplicate wells. *P < 0.05 for comparison of MRL.AND to control nonautoimmune transgenic strains. (B) The percentage of transgenic cells producing IL-2 was estimated using intracellular cytokine staining with anti–IL-2 by gating on CD4⁺Vα11^hi double-positive cells. *P < 0.05 for comparison of MRL.AND T cells to CBA.AND and B10.AND T cells.

MRL.AND transgenic T cells produce more IL-2 after stimulation with APLs than control transgenic T cells. (A) Negatively selected naive CD4⁺ AND transgenic T cells were stimulated in vitro with varying concentrations of a low affinity APL of PCC 88–104 (K99Q) presented by CH27 cells. IL-2 was determined by indirect ELISA from culture supernatant 24 h after stimulation. Error bars represent SEM of triplicate wells. *P < 0.05 for comparison of MRL.AND to control nonautoimmune transgenic strains. (B) The percentage of transgenic cells producing IL-2 was estimated using intracellular cytokine staining with anti–IL-2 by gating on CD4⁺Vα11^hi double-positive cells. *P < 0.05 for comparison of MRL.AND T cells to CBA.AND and B10.AND T cells.