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. 2001 Feb 5;193(3):307–316. doi: 10.1084/jem.193.3.307

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Predominantly monoallelic expression of Ly49A and Ly49G2. Freshly isolated, nylon wool nonadherent splenic cells from B6, BALB.B, and (B6 × BALB.B)F1 mice (all H-2^b) were harvested. Gated DX5⁺CD3⁻CD8⁻ NK cells were analyzed with (A) the Ly49A^B6 specific mAb, A1, versus the Ly49A^BALB specific mAb, TNTA, or (B) with Ly49G2^B6-specific mAb 3/25 versus 4D11, which recognizes both Ly49G2^B6 and Ly49G2^BALB alleles. Data represent means ± SD of three or four determinations on cells from individual animals.