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. 2001 Feb 5;193(3):307–316. doi: 10.1084/jem.193.3.307

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Independent allelic expression of Ly49A and Ly49G2. NK cells expressing Ly49G2^B6 only, Ly49G2^BALB only, or both Ly49G2^B6 and Ly49G2^BALB were sorted and expanded for 10–15 d in medium containing IL-2. (A) Reanalysis of the sorted populations with Ly49G2 mAbs demonstrated that they remained >89% pure after the culture. (B) Analysis of the sorted populations using allele-specific Ly49A mAbs. The experiment was repeated with NK cells from class I–deficient mice with similar results.