FIG. 3.

RT-PCR analysis of PLD transcription levels in gonococci was performed as described in the text. RNA was isolated from gonococci collected from 90-min and 3-h infections of PEX cells. Alternatively, RNA was harvested from gonococci in parallel (mock) experiments performed in the absence of PEX cells (control). These data demonstrate that transcription levels of PLD are not increased by 90 min but are increased by 3 h of infection.