TABLE 4.
PLD activity in cervical cells treated with cytoskeletal inhibitorsa
| Cell fraction | PLD activity in ectocervical cell fractions, mean fluorescence units (variance)
|
|||
|---|---|---|---|---|
| Wortmannin treatment
|
Cytochalasin D treatment
|
|||
| Membrane | Cytosolic | Membrane | Cytosolic | |
| Uninfected, untreated | 0.58 (0.04) | 0.39 (0.02) | 0.37 (0.04) | 0.48 (0.05) |
| Uninfected, treated | 0.47 (0.05) | 0.40 (0.01) | 0.39 (0.01) | 0.40 (0.03) |
| 1291, untreated | 0.99 (0.10) | 0.86 (0.08) | 1.04 (0.06) | 0.85 (0.09) |
| 1291, treated | 0.78 (0.06) | 0.41 (0.03) | 0.44 (0.03) | 0.46 ((0.03) |
| 1291ΔPLD, untreated | 0.41 (0.01) | 0.41 (0.02) | 0.34 (0.01) | 0.39 (0.01) |
| 1291ΔPLD, treated | 0.42 (0.02) | 0.41 (0.01) | 0.39 (0.01) | 0.33 (0.07) |
a
Cellular fractionation and PLD activity were assayed as described in the text. Data given are the mean values obtained from three trials in which gonococci were removed by filtration through a 0.22-mm syringe filter (see Materials and Methods). Positive and negative control values were 1.13 ± 0.29 and 0.18 ± 0.04, respectively.