TABLE 5.

Adherence to and/or invasion of primary cervical cells by gonococci in the presence and absence of PLD substrate competimers^a

Competimer	1291b		1291ΔPLD, % invasionc
	% Inhibition	% Invasion
None	NA	2.78 (0.16)	0.34 (0.02)
Ethanol
1.0%	90.73 (0.92) (P < 0.05)	0.26 (0.01) (P < 0.05)	0.33 (0.03) (P > 0.75)
0.1%	80.92 (1.41) (P < 0.05)	0.53 (0.01) (P < 0.05)	0.34 (0.04) (P > 0.90)
0.01%	74.96 (0.87) (P < 0.05)	0.70 (0.02) (P < 0.05)	0.34 (0.04) (P > 0.90)
2-Butanol
1.0%	ND	2.70 (0.47) (P > 0.90)	0.30 (0.05) (P > 0.25)
0.1%	ND	2.71 (0.47) (P > 0.75)	0.32 (0.03) (P > 0.25)
0.01%	ND	2.87 (0.53) (P > 0.75)	0.28 (0.02) (P > 0.25)
Phosphatyacholine (μg/ml)
10.0	98.78 (0.08) (P < 0.05)	0.03 (0.01) (P < 0.05)
1.0	92.47 (0.38) (P < 0.05)	0.20 (0.01) (P < 0.05)
0.1	87.36 (0.28) (P < 0.05)	0.34 (0.01) (P < 0.05)
0.01	68.81 (2.15) (P < 0.05)	0.84 (0.03) (P < 0.05)
0.0	NA	2.71 (0.11) (P < 0.05)

^aValues given are the mean values for percent invasion determined as a function of the original inoculum and the subsequent number of colony-forming units formed with subsequent plating of the ecto- or endocervical cell lysates. Inhibition values given were determined as a normalized function of the ability of the gonococcus to invade primary ectocervical cells in the presence of, in comparison to the absence of, alcohol or phosphatidylcholine competimers, as outlined in the text. Data given are the mean values obtained from at least three trials performed in triplicate. Variances are noted parenthetically. P values were determined with a Kruskal-Wallis k-sample analysis of variance. NA, not applicable. ND, not determined.

^bP values were calculated for wild-type gonococci in the presence of competimer compared to the absence of the competimer.

^cP values were calculated for PLD mutant gonococci in the presence of competimer compared to the absence of the competimer.