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. 2001 Sep 3;194(5):557–570. doi: 10.1084/jem.194.5.557

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Single cell analysis of J chain expression in secretory B cells from wt mice. (A) RT-PCR analysis of J chain and secretory versus membrane bound IgM expression in two different murine cell lines and in LPS-stimulated total splenocytes representing different differential stages of B cell development. 230–238 represents a pre-B, A20 represents a mature B cell, and the LPS-stimulated total spleen represents the plasma cell stage. (B) Summary of single cell RT-PCR analysis of J chain and secretory IgM transcripts in LPS-stimulated splenic B cells. (C) Example of experimental data showing one example of a single cell expressing only secretory IgM and of a single cell expressing both J chain and secretory IgM. HPRT is used as a cDNA control.