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. 2001 Sep 17;194(6):809–822. doi: 10.1084/jem.194.6.809

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Immunohistochemical localization of TGF-β₁. Immunohistochemistry was used to localize the sites of TGF-β₁ in WT transgene (−) mice and CC10-IL-13 transgene (+) animals (2–3 mo of age). Epithelial TGF-β₁ can be appreciated in the transgene (−) animals (A, original magnification: 20×). Macrophage TGF-β₁ is increased (C, original magnification: 20× and E, original magnification: 40×, black arrows), and alveolar type II epithelial cell (F, original magnification: 40×, white arrows) and eosinophil (F, 40×, black arrow) TGF-β₁ can be appreciated in IL-13 transgene (+) animals. In all cases, preincubation with TGF-β peptide abrogated TGF-β₁ staining (B and D, original magnification: 20×).