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. 2001 Sep 17;194(6):823–832. doi: 10.1084/jem.194.6.823

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Characterization of blood lymphocytes in CD25-depleted C57BL/6 mice. (A and B) Blood lymphocytes from a naive (A) or a CD25-depleted (B) mouse were stained 21 d after CD25 depletion, with APC-conjugated anti-CD4 and PE-conjugated anti-CD25. Numbers in the upper right quadrant indicate the percentage of CD25⁺/CD4⁺ T cells of total CD4⁺ T cells. (C and D) Blood lymphocytes from a naive (C) or a CD25-depleted/B16-GM-CSF/anti–CTLA-4 vaccinated mouse (D) were stained on day 17 after vaccination for the presence of TRP-2^180-188–specific CD8⁺ T cells using an APC-conjugated TRP-2^180–188 K^b-tetramer and anti–CD8-FITC. Numbers in the upper right quadrant indicate the percentage of TRP-2^180–188–specific CD8⁺ T cells of total CD8⁺ T cells. Representative stainings of three independent experiments are shown.