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. 2001 Sep 17;194(6):781–796. doi: 10.1084/jem.194.6.781

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© 2001 The Rockefeller University Press

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CD91 is involved in C1q- and MBL-mediated uptake via CRT. (A) CRT colocalizes with CD91 on the HMDM cell surface. HMDMs were stained with chicken anti–human CRT (anti–N-terminus, shown) and mouse anti–human CD91α and β (see Materials and Methods). Antibody staining was detected with FITC anti–mouse and Cy3 anti–chicken. n = 3, representative experiment shown. (B) Uptake of apoptotic cells is inhibited by antibody to CD91. HMDMs were preincubated with anti-CD91 antibody for 30 min at 37°C. Media was then aspirated and apoptotic cells were added for 1 h for a phagocytosis assay (see Materials and Methods). anti-CRT, chicken anti-CRT, NH₂ terminus; anti-CD91α, anti-CD91, α chain; anti-CD91β, anti-CD91, β chain. n = 3 ± SEM (control mean phagocytic index = 20.3 ± 1.5, P < 0.002). (C) C1q tail E_bab and α2m E_bab are taken up into HMDMs; this uptake is inhibited by anti-CD91 and by anti-CRT. E_bab coated with C1q tails, α2m, or BSA (not shown) were fed to HMDMs for 20 min at 37°C (see Materials and Methods). n = 4. P < 0.1 Engulfed; P < 0.1 Adherent.

CD91 is involved in C1q- and MBL-mediated uptake via CRT. (A) CRT colocalizes with CD91 on the HMDM cell surface. HMDMs were stained with chicken anti–human CRT (anti–N-terminus, shown) and mouse anti–human CD91α and β (see Materials and Methods). Antibody staining was detected with FITC anti–mouse and Cy3 anti–chicken. n = 3, representative experiment shown. (B) Uptake of apoptotic cells is inhibited by antibody to CD91. HMDMs were preincubated with anti-CD91 antibody for 30 min at 37°C. Media was then aspirated and apoptotic cells were added for 1 h for a phagocytosis assay (see Materials and Methods). anti-CRT, chicken anti-CRT, NH₂ terminus; anti-CD91α, anti-CD91, α chain; anti-CD91β, anti-CD91, β chain. n = 3 ± SEM (control mean phagocytic index = 20.3 ± 1.5, P < 0.002). (C) C1q tail E_bab and α2m E_bab are taken up into HMDMs; this uptake is inhibited by anti-CD91 and by anti-CRT. E_bab coated with C1q tails, α2m, or BSA (not shown) were fed to HMDMs for 20 min at 37°C (see Materials and Methods). n = 4. P < 0.1 Engulfed; P < 0.1 Adherent.