Table 1.
Characterization of Heteroclitic Analogs Identified from Tumor and Viral Antigens
| Antigen | Sequence | Heteroclitic substitution | Type of substitution | Position of substitution | Th1 cytokines | Th2 cytokines | A0201 binding (IC50 nM) |
|---|---|---|---|---|---|---|---|
| CEA.691 | IMIGVLVGV | None (WT) | None | 1 | 10 | 54 | |
| CEA.691M3 | IMMGVLVGV | I→M | Conservative | 3 | 10−5 | 1 | 27 |
| CEA.691H5 | IMIGHLVGV | V→H | Semiconservative | 5 | 10−7 | 10−1 | 16 |
| MAGEA3.112 | KVAELVHFL | None (WT) | None | 1 | NS | 94 | |
| MAGEA3.112I5 | KVAEIVHFL | L→I | Conservative | 5 | 10−4 | NS | 66 |
| MAGEA3.112W7 | KVAELVWFL | H→W | Semiconservative | 7 | 10−7 | NS | 7 |
| MAGEA2.157 | YLQLVFGIEV | None (WT) | 1 | 10 | 40 | ||
| MAGEA2.157I5 | YLQLIFGIEV | V→I | Conservative | 5 | 10−4 | 10−2 | 476 |
| MAGEA2.157F5 | YLQLFFGIEV | V→F | Semiconservative | 5 | 10−2 | 10−2 | 212 |
| HBV Pol.455 | GLSRYVARL | None (WT) | 10 | 10 | 83 | ||
| HBV Pol.455P7 | GLSRYVPRL | A→P | Conservative | 7 | 10−2 | 10−2 | 267 |
| HIV Pol.476 | ILKEPVHGV | None (WT) | >10 | >10 | 369 | ||
| HIV Pol.476H3 | ILHEPVHGV | K→H | Conservative | 3 | 1 | 1 | 78 |
| HIV Pol.476L3 | ILLEPVHGV | K→L | Semiconservative | 3 | 10−1 | 1 | 63 |
WT, wild-type.