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. 2003 Nov;71(11):6165–6170. doi: 10.1128/IAI.71.11.6165-6170.2003

FIG. 1.

FIG. 1.

Differential-display PCR analysis of uninfected and Rickettsia-infected ovary cDNA. RNA was isolated from uninfected and Rickettsia-infected ovaries and used for first-strand cDNA synthesis, and the cDNA was used as a template for differential-display PCR. Samples were electrophoresed on a 6% denaturing polyacrylamide gel, and the dried gel was exposed to film overnight at −80°C. U and I represent uninfected and Rickettsia-infected samples, respectively. Numbers identify clones with putative identifications as follows: 1, Oi312-SGS-3; 2, Oi411-PfX; 3, Oi616-Dreg-2; 4, Oi619-VASP; 5, Oi6113-clathrin-coated V-ATPase; 6, Oi812-α-catenin; 7, Oi814-GRP; 8, Oi1013-tubulin α-chain.