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. 2001 Sep 17;194(6):769–780. doi: 10.1084/jem.194.6.769

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© 2001 The Rockefeller University Press

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CD4⁺ T cells divide in response to antigen presented by DCs in vivo, produce IL-2 but not IFN-γ, and are then rapidly deleted. (A) CFSE labeled CD45.1⁺ 3A9 T cells were transferred into B10.BR and 24 h later, the recipients were injected subcutaneously in the footpads with αDEC/HEL (0.2 μg), GL117/HEL (0.2 μg), HEL peptide in CFA, or PBS. CD4⁺ T cells were purified by negative selection from regional LNs 3 d after challenge with antigen and analyzed by flow cytometry. The plots show staining with 1G12 anti-3A9 and CFSE intensity on gated populations of CD4⁺CD45.1⁺ cells. The numbers indicate the percentage of CFSE high (undivided) and CFSE low (divided) CD4⁺ T cells. The results are from one of two similar experiments. (B) T cells produce IL-2 but not IFN-γ in response to antigens presented on DCs under physiological conditions. 3A9 cells were transferred into B10.BR mice and 24 h later the recipients were injected subcutaneously in the footpads with αDEC/HEL (0.2 μg), GL117/HEL (0.2 μg), HEL peptide in CFA. CD4⁺. Histograms show staining with anti–IL-2 and anti–IFN-γ on gated populations of 3A9⁺CD4⁺ cells. The thick lines indicate PBS control. (C) Same as in panel A but analysis performed 7 or 20 d after antigen administration.

CD4⁺ T cells divide in response to antigen presented by DCs in vivo, produce IL-2 but not IFN-γ, and are then rapidly deleted. (A) CFSE labeled CD45.1⁺ 3A9 T cells were transferred into B10.BR and 24 h later, the recipients were injected subcutaneously in the footpads with αDEC/HEL (0.2 μg), GL117/HEL (0.2 μg), HEL peptide in CFA, or PBS. CD4⁺ T cells were purified by negative selection from regional LNs 3 d after challenge with antigen and analyzed by flow cytometry. The plots show staining with 1G12 anti-3A9 and CFSE intensity on gated populations of CD4⁺CD45.1⁺ cells. The numbers indicate the percentage of CFSE high (undivided) and CFSE low (divided) CD4⁺ T cells. The results are from one of two similar experiments. (B) T cells produce IL-2 but not IFN-γ in response to antigens presented on DCs under physiological conditions. 3A9 cells were transferred into B10.BR mice and 24 h later the recipients were injected subcutaneously in the footpads with αDEC/HEL (0.2 μg), GL117/HEL (0.2 μg), HEL peptide in CFA. CD4⁺. Histograms show staining with anti–IL-2 and anti–IFN-γ on gated populations of 3A9⁺CD4⁺ cells. The thick lines indicate PBS control. (C) Same as in panel A but analysis performed 7 or 20 d after antigen administration.